Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Functional analysis of cells stimulated with CD154. (A) Viability assay. No significant changes were observed in all four cell lines. (B) Migration assay. (C) Invasion assay. Migration and invasion were enhanced in TE-5 and TE-10 cells, which highly expressed CD40, after rsCD154 stimulation. No significant changes were observed in low CD40 expression cells (TE-4 and −11 cells). The Mann-Whitney U test was performed using GraphPad Prism 9 software. ns, not significant; OD, optical density; rsCD154, recombinant soluble CD154.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques: Functional Assay, Viability Assay, Migration, Invasion Assay, Expressing, MANN-WHITNEY, Software, Recombinant

Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Changes in gene expression caused by the CD40-CD154 interaction in TE-10 cells. Screening of 40 genes involved in extracellular matrix remodeling. The expression ratios of target genes relative to GAPDH are shown on the left. On the right, CD154-induced changes in gene expression are displayed in descending order. Upregulation of gene expression in the order MMP-9 > PLG > LAMC2 was confirmed following rsCD154 stimulation.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques: Gene Expression, Expressing

Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Changes in MMP-9 expression caused by the CD40-CD154 interaction in TE cells. (A) Qualitative analysis of MMP-9 secretion from TE cells using gelatin zymography. Increased MMP-9 secretion was observed in high CD40 expression cells (TE-5 and −10), regardless of the baseline MMP-9 secretion level without CD154 stimulation. (B) Quantitative analysis of MMP-9 secretion from TE cells using an ELISA. Increased MMP-9 secretion was observed in TE-5 and −10 cells. (C) Quantitative analysis of MMP-9 mRNA expression in TE cells via reverse transcription-quantitative PCR. MMP-9 mRNA expression upregulation was observed in high CD40 expression cells (TE-5 and −10). The Mann-Whitney U test was performed using GraphPad Prism 9 software. n.d., not detected; rsCD154, recombinant soluble CD154.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques: Expressing, Zymography, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Software, Recombinant

Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Experiments using CD40-knockdown TE-10 cells using siRNA. (A) Transfection with CD40 siRNA resulted in a reduction in CD40 mRNA expression. The average relative CD40 mRNA expression was compared using GraphPad Prism 9 software. After one-way ANOVA, Bonferroni post hoc analysis was performed. (B) CD154 stimulation of CD40 knockdown TE-10 cells was performed, and MMP-9 upregulation was suppressed. (C) Migration assay. CD40 knockdown reduced cell migration, and no increase in migration was observed following CD154 stimulation. The images on the right present a visualization of migrating cells under a 1.25× optical microscope, with the cellular regions indicated by green labelling. (D) Invasion assay. Similar to the migration assay, a decrease in invasion was observed following CD40 knockdown. The images on the right present a visualization of migrating cells under a 1.25× optical microscope, with the cellular regions indicated by green labelling. siRNA, small interfering RNA.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques: Knockdown, Transfection, Expressing, Software, Migration, Microscopy, Invasion Assay, Small Interfering RNA

Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Survival analysis of patients who underwent esophagectomy was performed using the Kaplan-Meier method. The log-rank (Mantel-Cox) test was conducted using GraphPad Prism 9 software. (A) Association between DFS and MMP-9 levels. (B) Association between DFS and CD154 levels. (C) Association between OS and MMP-9 levels. (D) Association between OS and CD154 levels. DFS, disease-free survival; MST, median survival time; OS, overall survival.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques: Software

Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Survival analysis of patients with esophageal cancer stratified by disease stage. (A) Association between DFS and MMP-9 levels in patients with pathological stage I. (B) Association between DFS and CD154 levels in patients with pathological stage I. (C) Association between OS and MMP-9 levels in patients with pathological stage I. (D) Association between OS and CD154 levels in patients with pathological stage I. (E) Association between DFS and MMP-9 levels in patients with pathological stage II–IV. (F) Association between DFS and CD154 levels in patients with pathological stage II–IV. (G) Association between OS and MMP-9 levels in patients with pathological stage II–IV. (H) Association between OS and CD154 levels in patients with pathological stage II–IV. Survival analysis was performed using the Kaplan-Meier method, and the log-rank (Mantel-Cox) test was conducted using GraphPad Prism 9 software. DFS, disease-free survival; MST, median survival time; OS, overall survival.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques: Software

Journal: Oncology Reports

Article Title: Platelets and MMP?9 contribute to esophageal cancer invasion via CD40?CD154 interactions

doi: 10.3892/or.2025.8912

Figure Lengend Snippet: Schema of the CD40-CD154 interaction in the tumor microenvironment of esophageal cancer. In tumor cells, the CD40-CD154 interaction induces the secretion of molecules involved in ECM remodeling, including MMP-9, contributing to cell invasion, EMT and metastasis. In immune cells, the CD40-CD154 interaction is involved in antitumor immunity, leading to cancer cell death. ECM, extracellular matrix; EMT, epithelial-mesenchymal transition.

Article Snippet: CD154 , REA238 , FC , 1:50 , Miltenyi Biotec GmbH.

Techniques:

Journal: PLoS ONE

Article Title: Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis

doi: 10.1371/journal.pone.0183214

Figure Lengend Snippet: CD4(+)/CD8(+) T-cells were isolated from tonsils of the different disease scenarios and their activation status was determined via flow cytometry analysis of the mixed CD4(+)CD8(+) preparations. (A) CD69, CD25 and CD154 activation marker surface expression determined by flow cytometry and plotted as percentage of all T-cells. (B) Double immune-staining for PD-1 and CD69 in all T-cells. Only statistically significant groups are labelled (* p<0.05). CT = chronic tonsillitis; PTA = peritonsillar abscess; HY = tonsillar hyperplasia; ton = tonsil; abs = abscess.

Article Snippet: Antibodies for flow cytometry analysis: anti-human CD3-FITC (clone MEM-57), anti-human CD4-PE (clone MEM-241), anti-human CD8-APC (clone MEM-31), anti-human CD45RA-FITC (clone HI100) and anti-human CD62L-APC (clone LT-TD180) from ImmunoTools (Friesoythe, Germany); Anti-human CD152 (CTLA-4)-PE (clone 14D3) and anti-human CD279 (PD-1)-FITC (clone MIH4) from eBioscience Inc. (San Diego, USA); CD25-APC human (clone 4E3), CD69-APC human (clone FN50), CD154-APC human (clone 5C8), anti-FoxP3-PE human (clone 3G3) and anti-IL-2-PE human (clone N7.48A) were purchased from Miltenyi Biotec GmbH (Bergisch Gladbach, Germany).

Techniques: Isolation, Activation Assay, Flow Cytometry, Marker, Expressing, Staining

Journal: PLoS ONE

Article Title: Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis

doi: 10.1371/journal.pone.0183214

Figure Lengend Snippet: CD4/CD8 T-cells isolated from tonsils were challenged with the indicated formulations of anti CD3 and/or CD28 Abs or with a mix of phorbol ester and Ionomycine (TPA/Iono) and subjected to flow cytometric determination of activation marker CD69 (A) , CD25 (B) and CD154 (C) expression, always plotted as percentage of all T-cells. All measurements were carried out on the mixed CD4(+)CD8(+) preparations. Representative fluorescence profiles for stimulated (black line) versus non-stimulated samples (grey curves) are shown on the left side of each panel. Beads: anti-CD3 and anti-CD28 Abs immobilized on beads. CT = chronic tonsillitis; PTA = peritonsillar abscess; HY = tonsillar hyperplasia; ton = tonsil; abs = abscess.

Article Snippet: Antibodies for flow cytometry analysis: anti-human CD3-FITC (clone MEM-57), anti-human CD4-PE (clone MEM-241), anti-human CD8-APC (clone MEM-31), anti-human CD45RA-FITC (clone HI100) and anti-human CD62L-APC (clone LT-TD180) from ImmunoTools (Friesoythe, Germany); Anti-human CD152 (CTLA-4)-PE (clone 14D3) and anti-human CD279 (PD-1)-FITC (clone MIH4) from eBioscience Inc. (San Diego, USA); CD25-APC human (clone 4E3), CD69-APC human (clone FN50), CD154-APC human (clone 5C8), anti-FoxP3-PE human (clone 3G3) and anti-IL-2-PE human (clone N7.48A) were purchased from Miltenyi Biotec GmbH (Bergisch Gladbach, Germany).

Techniques: Isolation, Activation Assay, Marker, Expressing, Fluorescence